3D-Electron Microscopy

Electron microscopy (EM) revolutionized biological research by providing high resolution micrographs of cellular ultrastructure and their interactions with other cells. Conventional 2D-EM has substantial limitations and manual serial sectioning approaches (to achieve 3D in EM resolution) are labor intensive which constrains its utility. With recent advances in technology and methods we make 3D-EM routinely possible.

We offer a complete range of automated EM imaging services and customizable analysis for biological research and drug development. Utilizing a technique called serial block-face scanning electron microscopy (SBFSEM) we generate a cost-effective, aligned stack of hundreds of serial sections overnight at nanoscale resolution.

Below is a brief comparison of our EM imaging specifications to other imaging technologies:

Our current 3D-EM services include:

Please find links to a few publications by our collaborators

Hematopoietic stem cell arrival triggers dynamic remodeling of the perivascular niche (Cell, 2015)

Nuclei-specific differences in nerve terminal distribution, morphology, and development in mouse visual thalamus (Neural Development, 2014)

An enteroendocrine cell – Enteric glia connection revealed by 3D electron microscopy (PLOS|One, 2014)

Semaphorin 5A inhibits synaptogenesis in early postnatal- and adult-born hippocampal dentate granule cells (Elife, 2014)

Finite element analysis of the pressure-induced deformation of Schlemm’s canal endothelial cells (Biomechanics and Modeling in Mechanobiology, 2014)

Astrocytes mediate synapse elimination through MEGF1- and MERTK pathways (Nature, 2013)

Dark side illuminated: imaging of Toxoplasma gondii through the decades (Parasites & Vectors, 2013)

WDR81 is necessary for Purkinje and photoreceptor cell survival (The Journal of Neuroscience, 2013)