OPC proliferation assay
This assay is used to identify compounds that change the rate of OPC proliferation. In this assay, OPCs are cultured with or without compounds in proliferation media containing thymidine analog EdU (5-ethynyl-2´-deoxyuridine) in 96-well plates for 1 to 24 hrs. EdU is incorporated into the DNA of proliferating cells and can be detected with ‘click’ chemistry. Following fixation and nuclear staining, cells are imaged and analyzed in a high-content ArrayScan™ reader. The percentage of cells that have incorporated EdU during specific time frames can then be compared.
Applications of the OPC proliferation assay include:
- Determination of possible effects of compounds on OPC proliferation.
- Determination of possible effects of compounds on OPC differentiation.
- Interactions of compounds on OPC proliferation.
Proliferating OPCs can be identified and quantified: Cells were imaged in a high-content ArrayScan reader with a 10x objective. Green, cells that have incorporated EdU within a specific period of time; blue, Hoechst-stained nuclei.