OPC and oligodendrocyte toxicity assay
The assay is used to identify the toxicity of compounds on OPC or oligodendrocytes. In this assay, cells are treated with or without compounds for a specific period of time in 96-well plates. At the end of the incubation period, specific different color fluorescent dyes are used to identify live and dead cells. Cells are imaged and analyzed in a high-content ArrayScan™ reader. The percentages of live and dead cells in each treatment conditions can then be compared. The assay provides more accurate measurement of live and dead cells than trypan blue exclusion, colorimetric, or morphology-based assays.
Applications of the OPC toxicity assay:
- Screening compound library for OPC or oligodendrocytes cytotoxicity.
- Determination of toxic concentration of lead compounds.
- Potential synergistic toxicity of compound combinations.
Viable and dead cells in the assay can be identified and quantified: Cells were images in a high-content ArrayScan reader with a 10x objective. Green, live cells; red, dead cells.